Molecular structure, DNA binding mode, photophysical The emission maximum is ~ nm. SYBR® Gold stain is >fold more sensitive than ethidium bromide for detecting DNA and RNA in denaturing urea, glyoxal, and formaldehyde gels, even with nm transillumination.1 For detecting glyoxalated RNA, SYBR® Gold stain is 25– times more sensitive than ethidium bromide (Figure 2) and is.
Product Information - ABP Bio SYBR™ Gold Nucleic Acid Gel Stain is the most sensitive fluorescent stain we offer for the detection of nucleic acids. Use with UV transilluminators, the Dark Reader (see stained nucleic acid gel below), laser scanners, or blue light transilluminators such as the Safe Imager™ or the E-Gel™ Imager. Features of SYBR™ Gold Nucleic Acid.
Oxazole Gold (SYBR® Gold), 10,000X in DMSO - Biotium SYBR Gold has two fluorescence excitation maxima when bound to DNA, one centered at ~ nm and one at ~ nm, and a single emission maximum at ~ nm. SYBR Gold exhibits improved DNA cloning efficiency compared to others dyes of the SYBR family because it can be excited with blue light transillumination, which does not cause DNA damage. [4].
SYBR Gold - Wikipedia Improved DNA Cloning Efficiency: Excitable with blue light transillumination, which does not cause DNA damage Increased Signal to Background Ratio: More than fold signal enhancement when bound to nucleic acids Versatile: Detects dsDNA, ssDNA and RNA in native, glyoxal, formaldehyde or urea gels.
Characterization of SYBR Gold Nucleic Acid Gel Stain: A Dye Variation in signal may be observed between longer and shorter oligos, however the overall linear range of the assay holds. The excitation maxima for Sybr® Gold is nm and the emission maxima is ~ nm. Assay Supplies Equipment: − NanoDrop Fluorospectrometer − 2 uL pipettor − Low retention nuclease free pipette tips Materials.
Helixyte Gold (SYBR Gold) - AAT Bioquest
SYBR Gold is a fluorescent compound with an excitation peak at nm and an emission peak at nm. It can be excited using a nm laser paired with a /40 nm bandpass filter, a configuration that can be found, for example, in the BD FACSJazz™.
SYBR Gold Nucleic Acid Gel Stain - Thermo Fisher Scientific Following brief incubation with the SYTOX Green stain, dead cells fluoresce bright green when excited with the nm spectral line of the argon-ion laser or with any other – nm source. Because all of the SYTOX dyes are essentially nonfluorescent in aqueous medium, no wash steps are required.
SYBR™ Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) Improved DNA Cloning Efficiency: Excitable with blue light transillumination, which does not cause DNA damage Increased Signal to Background Ratio: More than fold signal enhancement when bound to nucleic acids Versatile: Detects dsDNA, ssDNA and RNA in native, glyoxal, formaldehyde or urea gels.